You can also use it to understand why the body can't absorb minerals present in some foods if an ion such as iron or calcium is "salted out" in the digestive system, the body won't be able to absorb it very efficiently. For example, you can use this trick to remove heavy metal ions from wastewater. It has a number of important applications. It's also called "salting out" a precipitate. Your experiment illustrates the common ion effect: forcing an ionic compound to precipitate from solution by adding one of its ions to the solution. For example, the ion product we calculated in the previous step is less than K sp (4.4 × 10 -6 < 6.5 × 10 -6), so we wouldn't expect any precipitate to form in the tank. If the ion product is less than or equal to the K sp, no precipitate will form. If the ion product is greater than the K sp, the solution is supersaturated, and precipitate will form. For example, if the solution in the tank were 0.010 M Ca(OH) 2 and 0.001 M NaOH, we have:
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#Switch bear and breakfast Pc#On the same day, the PC version will receive gamepad support. Developed by Gummy Cat and published by Armor Games Studios, Bear and Breakfast will be released for Nintendo Switch on September 15. Juggling fuel, cooking, and other management gets overwhelming quickly, but luckily if you revisit your friends, like Julia the goat chef, they’ll offer you a quick quest. We hope everyone has a fun and relaxing time hanging out with the bear on the Switch. #Switch bear and breakfast how to#You’ll learn how to cook to appease your foodie guests, as well as fuel a heater to keep cold guests warm. Revisit the friends you made along the wayĪs you unlock more properties, they’ll require more to keep guests happy. You’ll unlock the map shortly after talking to Sabine, the local park ranger. You should use the map to scout out metal scrap, like broken washing machines and the like, to find quest items quickly. Resources respawn after about 24 in-game hours, and your map will show you where each item is. If the quest doesn’t have built-in navigation, the journal will essentially tell you where to exactly find the items you need. If you’re still stuck on finding quest items or finding NPCs, double check your quest journal. Image: Gummy Cat/Armor Games via Polygon Double-check your journal when you’re stuck Make a beeline and search through all the scrap you see to find these quickly. The story quests will often require you to find things like a microphone or satellite piece, which you will find in piles of scrap sitting around the map. You have tons of inventory space, along with a gigantic universal storage that you get access to early on and an eventual additional inventory upgrade. Pick up everything in Bear and Breakfast. Search everything, especially scrap piles The signs will offer you rewards (including quality-of-life improving items) in exchange for upgrading your properties, but you’ll often get gated by items you need to unlock via story. Focus on the story questsĪll that being said above, you’ll want to focus more on the story rather than the maintenance quests you get at sign posts. Peep the announcement and gameplay trailer below. The management adventure game saw a delayafter developer Gummy Catsaid they needed to tinker with controller play for a while longer, but it’s been out on PC via Steamsince July 28th. Lots of quest completion will end up getting locked behind story completion anyway, so don’t stress too much about getting them all done as soon as possible. Bear and Breakfastfinally has a release date on Nintendo Switch: September 15th. Focus on one quest (probably your story quest) at a time and go back for the others when you can. Our Bear and Breakfast beginner’s guide will provide tips that we wish we had when starting out.Īs you unlock more locations like the desert or snowy mountains, you’ll get more quests, but it’s important to take it a step at a time to avoid being overwhelmed. #Switch bear and breakfast windows#While the game is marketed as a cozy indie, things can get hectic quickly depending on how you play. The hotel management game Bear and Breakfast has some fans waiting for the delayed Switch release, and they finally have a date. Bear and Breakfast first released back in July on Windows PC via GOG and Steam, and will be coming to Nintendo Switch on September 15th.The PC version of the game will also be getting controller. #Switch bear and breakfast download#Not everyone is as helpful or as needy though.Bear and Breakfast is the adorable management sim for PC where you play as a bear named Hank trying to restore life and tourism to his area. Publisher: Armor Game Studios Download size: 1.058 GB ESRB Rating: Everyone 10+ Play modes: TV, Tabletop, Handheld Languages: Japanese, English. Most of them have something to say about your endeavors and if you take some time out of your day to listen, they might help your build your shack to new heights! Moving through the story-rich world of Bear and Breakfast will have you stumbling on dozens of interesting folks and weirdos, much like yourself. Uncover a world rich in lore, side quests, characters, and secrets. Complete quests and story lines to collect new items and perks for your inn. Keep 'em happy to maintain your reputation, earn money and attract new customers! Bring guests into the forest to stay at your inn. Each room can be individually customized completely from furniture to fixtures. Build and personalize your inn with dozens of guest rooms, bathrooms, parlors, and entertainment. As your business expands so do the mysteries of the forest, and Hank soon finds himself uncovering a plot deeper than the wilderness itself. Hank and his friends find an abandoned shack and, equipped with their teenage ingenuity, turn it into a money-making bed and breakfast scheme for unsuspecting tourists. Bear and Breakfast is a laid-back management adventure game where you play as a well-meaning bear trying to run a B+B in the woods. Holly Hughes of the Reiger Memorial Library in Haskell agrees, citing how libraries have long been places for people to access information and technology that they might not otherwise be able to obtain. “We hope the hotspot program will fill a digital gap for the households that currently lack access to online resources for job training, educational research and social connections.” “We know many local residents use the library exclusively for their internet connectivity,” said Alison Bloyd of the Thomas-Wilhite Memorial Library in Perkins. Whitacre hopes this pilot program will lead to more libraries across the state embracing the idea of providing mobile hotspots and beginning lending programs of their own. The pilot program will run through April 2018 and is being supported by Mobile Beacon, an organization focused on providing broadband to nonprofit entities across the nation. Generally speaking, most libraries with such a program allow adults with a valid library card to check out the devices. Individual libraries have specific policies about who can check out the hotspot devices and the length of the loan period. The hotspot technology can be used to hook up multiple devices. As long as the cellular network provider used by the hotspot has service in that area, the devices will provide broadband access.” “They can be used inside a home taken to restaurants, community centers and the like and even go on a road trip. “These devices use cellular networks, the same as smartphones,” Whitacre said. The program essentially “loans out the internet” by allowing individuals to check out one of four mobile hotspot devices assigned to the library. This is less than half of the 91 percent for Oklahoma households with incomes greater than $100,000.Įnter the pilot program between DASNR and the public libraries. Life in the 21 st century has increasingly become interdependent with internet access, yet the cost of maintaining a monthly connection exceeds what far-too-many Oklahomans can afford.Ī 2015 survey indicated that only 44 percent of Oklahoma households with incomes of less than $25,000 have a broadband connection. “It will enable kids to be able to do their web-based homework, adults to do job applications or patrons to just cruise the internet.” “As a small rural library with many areas not having internet access, I hope this will provide yet another beneficial service to our communities,” said Leslie Durham of the Elgin Community Library. “This pilot program is something that has never been done in rural Oklahoma and hopefully will allow residents and civic leaders to experience firsthand how broadband access can improve people’s quality of life and strengthen local communities,” said Brian Whitacre, Oklahoma State University Cooperative Extension agricultural economist and the program’s principal investigator.ĭASNR and its cooperating partners – the Elgin Community Library, the Reiger Memorial Library in Haskell, the Thomas-Wilhite Memorial Library in Perkins and the Seminole Public Library – are asking local residents to take advantage of the pilot program and provide feedback as to their experiences. We quantitated the amount of DegP on a Western blot using cells grown at 44☌ expressing wild-type degP and degPS210A from pCS20 and pCS21, respectively, induced with 10 and 50 μM IPTG. However, compared to wild-type degP, a higher level of degPS210A expression was required for complementation. Colony formation of degP null mutants expressing degPS210A at 44☌ indicated that the chaperone activity of DegP was sufficient to rescue the cell from thermal folding stress ( Figure 7). If the chaperone activity of DegP is sufficient to reduce folding stress caused by elevated temperatures, the expression of degPS210A should suppress the temperature sensitivity of degP null strains. As expected, DegP S210A did not degrade denatured MalS.Īdditional in vivo evidence for the general molecular chaperone activity of DegP was obtained by investigating the T s phenotype of degP null mutants. At 28☌, incomplete degradation of MalS was observed that was in agreement with the lower protease activity detected when using resorufin-labeled casein as a substrate. As documented for selected samples ( Figure 4B), wild-type DegP degraded MalS at 37☌ and 42☌. Essentially the same results were obtained as for resorufin-labeled casein. To further investigate the temperature dependence of the proteolytic DegP activity and to verify that DegP S210A is indeed proteolytically inactive, we coincubated denatured MalS and DegP under reducing conditions, in the presence of 30 mM DTT, over the whole temperature range. This finding explained the temperature-dependent degradation of MalS in the in vitro refolding assays ( Figure 3B). At 28☌, DegP was 8-fold less active than at 42☌, and a major increase in proteolytic activity was observed between 32☌ and 42☌ ( Figure 4A). Below 22☌, DegP was proteolytically inactive. Proteolytic activity was assayed between 4☌ and 42☌, using purified DegP. To obtain quantitative data for the temperature dependence of the proteolytic DegP activity, we used resorufin-labeled casein as a substrate. So far our results have indicated that wild-type DegP has molecular chaperone activity at low temperatures and proteolytic activity at elevated temperatures. The explanation that DegP could first refold and subsequently degrade properly folded MalS can be excluded, since we detected that properly folded MalS was no substrate of DegP (data not shown). At 42☌, the amount of oxidized MalS was the same in samples containing glutathione and wild-type DegP, respectively, suggesting that wild-type DegP did not refold MalS under these conditions. These results indicated that wild-type DegP has chaperone activity at low temperatures and protease activity at elevated temperatures. In the presence of wild-type DegP, degradation of reduced MalS was observed and the total amount of MalS was lower compared to samples containing DegP S210A ( Figure 3B). At 37☌ and 42☌, DegP S210A also stimulated refolding of MalS. The addition of equimolar concentrations of BSA did not stimulate refolding of MalS ( Figure 3A). At 28☌, both DegP and DegP S210A stimulated refolding of MalS more efficiently than glutathione. DegP and DegP S210A were directly added to the refolding assays. We carried out refolding of reduced and denatured MalS in the presence of purified wild-type DegP and the proteolytically inactive DegP S210A mutant ( Figure 3). Refolding Assay of Reduced and Denatured MalS In dsbA degP double null mutants expressing degPS210A, MalS activity was increased 11-fold over the dsbA degP double mutant background ( Table 1). In dsbA degP double null mutants, MalS activity was 30-fold lower as compared to wild-type strains and 18-fold reduced as compared to dsbA mutants. These results were supported by assaying amylase activity. In this strain, a fraction of MalS could fold into the oxidized and soluble form, since it fractionated with the supernatant of shocked cells. To obtain further evidence for a potential chaperone activity of DegP, we expressed a proteolytically inactive variant of degP ( degPS210A) in dsbA degP double null mutants. These results indicated that DegP may be involved in the folding of MalS in dsbA mutants at low temperatures. As expected, misfolded MalS was found in the pellet fraction of shocked cells. In dsbA degP double null mutants, only reduced MalS was detected. This was in agreement with the previous finding that nearly 60% of MalS activity was detected in dsbA mutants in comparison to wild-type cells after growth at 28☌ ( Table 1). In dsbA mutant background, most of the oxidized form of MalS was present in the supernatant, and smaller amounts of oxidized and reduced MalS were found in the pellet fraction. In agreement with this fact, properly folded MalS was released by cold osmotic shock from wild-type cells. If you take a date, you may wish you didn’t have to share! The atmosphere was intimate but busy- just how I like it. Everything I ate was exquisite, but if you only order one thing, make sure it is the tuna pizza! Each dish was not only mouthwateringly delicious, it was a work of art. This is definitely a firm favourite from the Yellowtail menu. What I thought: The Big Eye Tuna Pizza…wow. What I ate: I had the Big Eye Tuna Pizza with Micro Shiso and Truffle Oil, the Kobe Beef Tataki with Spicy Daikon, Garlic and Maldon Sea Salt, seared scallops topped with caviar, Lobster carpaccio with Sweet Shaved Onion, Cilantro and Amazu Ponzu, and Tempura Alaskan King Crab with Serrano, Sweet Ponzu and Shaved Red Onion. In addition to mouth-watering sushi, guests are also treated to a fantastic view of the Bellagio Fountain Show. This upscale sushi restaurant serves a menu of authentic traditional and modern Japanese cuisine created by award-winning Chef Akira Back. The Lowdown: Yellowtail at Bellagio was highly recommended by everyone I met in Las Vegas, and it certainly didn’t disappoint. *Warning…this might make you hungry* Yellowtail at Bellagio After 7 days of sushi, I’m surprised I didn’t turn into a fish! So here it is…a list of some of the best sushi on the Las Vegas Strip. I spent a week in search of Vegas’s finest sushi restaurants, sampling dishes from simple nigiri and sashimi, to exquisite Japanese tapas and innovative sushi rolls bursting with flavour. Now you might be thinking “Wait a second…Vegas is in the middle of the dessert, right?” I am often a little bit skeptical about eating fish when I am nowhere near the water, but the fish and seasonal ingredients are flown in daily from the finest suppliers in the world. So you can imagine how excited I was to feed my addiction for raw fish and check out the Strip’s sushi offerings. There are restaurants to satisfy any food craving in Las Vegas, including sushi. Las Vegas these days isn’t just about gambling, shows and partying…it’s also foodie heaven. It’s all about culinary experiences, and the Strip is now home to some of the world’s most celebrated chefs. Wherever I travel to, I seem to end up eating sushi at some point or another during my trip. If I had to choose only one type of food to eat for the rest of my life, it would be sushi. Want to know where to find the the best sushi in Las Vegas? Here are my top restaurant picks on the Strip… Web cam software to manipulate web cam stream. Show text and other information on the web cam. Change your frame color: In this new window, locate the Hue Shift slider. Select ‘Image Mask/Blend’ Click the ‘+’ located under the ‘Effects Filters’ section. Right click on the layer you wish to apply a filter to then select ‘Filters’. In the next window, click the + icon, then select Color Correction from the Filter Type menu. Add two video sources Make sure the video layer you want to use an image mask on is the top layer. There are thousands of special effects to choose between. Add a color correction filter: Back in your Sources tab, right-click your new webcam frame source, then select Filters from the dropdown. public float DistanceUp //how high the camera is above the player. Code (CSharp): Header ('Camera Properties') public float DistanceAway //how far the camera is from the player. There are six view ports which can each be assigned a different special effect which can create a kaleidoscope of visions of what the web cam sees. I know a lot of other people are in need of a dynamic camera so its only right that I share this script. Cammask change my video cam driver#The virtual web cam driver ensures that the web cam can be inserted into a number of different applications. " CamMask add effects into webcam pictures"ĬamMask is a web cam application to complement the web cam with new features like video output, image gallery and chatting screen. Darla says, "Each painting is an adventure I can't wait to begin," and her favorite part of creating is seeing a blank canvas as the opportunities are endless and that is when the fun commences. #PACIFIC NORTHWEST ART PROFESSIONAL#She became a professional artist in 2005 after first starting to paint in 2004. Oregonian Darla Kay Boljat grew up in Tigard and currently calls Sherwood her home. I'm a member of a local critique group whose members use varied media and we continue to experiment and grow in terms of technique, composition, color, and design. Since retirement in 2000, I've 'gone to my room' and practiced to find my own voice with paint and colors. I've been drawing and painting since grade school and took classes at local art schools, art centers and community college in the 70's and 80's in the Portland area, moonlighting while I was employed. I am presently focused on these two mediums. Although I am predominantly a representational landscape and nature painter, I also enjoy experimenting with collage and acrylic stained papers in abstract & figurative works. I enjoy applying and scraping pigment with knives to achieve interesting texture and tones. On the computer, I might convert the photo to black & white to just see values, and blur it (instead of squinting), then let my intuition guide the color choices, trying to remain as free as possible with the paint application via fluid negative brush painting or bold palette knife strokes, always conscious of the of light against dark…I am so drawn to light and shadows. I am usually inspired by my own photos taken at local areas, zooming in on a particular portion of the scene where I find a pleasing composition & design. My art is an emotional response to color, texture and design in nature.
Consider an object contaminated if you have any doubt as to its sterility Consider the edge (outer 1 inch) of a sterile field to be contaminated Compare and contrast medical and surgical asepsis. skin#All items brought into contact with broken skin or used to penetrate the skin in order to inject substances into the body, or to enter normally sterile body cavities, should be sterile Never walk away from or turn your back on a sterile field Avoid talking, coughing, sneezing, or reaching over a sterile field or objectį. Hold sterile objects above the level of the waistĮ. Avoiding spilling any solution on a cloth or paper used as a field for a sterile set-upĭ. Open sterile packages so that the first edge of the wrapper is directed away from the worker to avoid the possibility of a sterile wrapper touching unsterile clothingĬ. Only a sterile object can touch another sterile objectī. Basic principles of Surgical Asepsis include:Ī. An object or area is described as being sterile or not sterile. Compare and contrast medical and surgical asepsis. free#Surgical Asepsis also known as Sterile Technique requires strict adherence to ordered and specific procedures which render an area free from all microorganisms including spores.
Our first, and highly successful, Directors Toolkit! New public library directors quickly learn what seasoned directors already know: running a library means youve always got your hands. Art directors toolkit software#Code Line is a proven developer of software geared toward improving the workflow of Graphic Artists. Art Directors Toolkit Directors Toolkit Download. Upgrades from previous versions are available for 19.95 USD. Licenses can be purchased online for 39.95 USD. Jason is a great communicator and can pass on the experience he has has to people in a warm and non-threatening way.”ĭay Two ended with a discussion over drinks as the participants questioned Jason and the actors, seeking to draw out as much wisdom as possible. Art Directors Toolkit is available as a free, 15-use demo from Code Line's website. I felt very free to question and participate in the workshop. “This was a very valuable experience and I learnt a lot. Art directors toolkit how to#The afternoon was spent working with talented actors Steven Lovatt, Jeff Szusterman and Rene Lyons as Jason worked through blocking, coverage further techniques on how to get the best performance.įor the directors attending, the benefits were immense: Having just returned from the American Film market where he pitched his film in post production and his next planned feature, Jason gave everyone the benefit of his market wisdom and knowledge, particularly on the US film making scene. On Day Two, Jason introduced further techniques he uses with actors to develop connection, before moving on to visual effects, technical considerations and the importance of Art Department, Costume, Makeup and other tools that build character. To illustrate some of his points, he engaged participants in improvisation, and gave his thoughts on rehearsing and how to deal with exposition.Įxperienced actors Nicola Kawana and Steven Papps joined the class in the afternoon, when Jason ran through his blocking technique and how he moves actors to final performance using a scene from a highly acclaimed TV drama. On Day One, Jason spoke at length on his method, reveal tips that work for him to get connection between actors. Jason is a proponent of the Steven Spielberg-style, and covered the master shot method he uses to get fast, efficient and effective wide shots that deliver coverage with little need for relighting. Last weekend independent filmmaker Jason Stutter of Tongan Ninja, Predicament and Careful With That… entranced 10 budding drama directors with his practical approach to directing film. Originally the Joinme software had me check the option USB debugging under developer options, I have tried unchecking this and going back to checked as it had me set up with no solution to the problem.Īlso when first connected the phone installed the Joinme software/drivers it will no longer do that or give me that option. And an now signalizing the Ships at Plymouth to join me orphan child of. #Joinme zte install#from charge only, install driver, media device (mtp) * (this is the option that was working perfectly and still on the rare occasion it wants to), to camera (ptp). I have changed every available setting dealing with usb or pc connectivity that I can find. Contacted (well tried to) ZTE no help, nothing, nada, zip! requested help from Microsoft neither their tech nor myself could find any issues that may be causing this.Ĥ. Downloaded any Dell updates, bios updates, and installed as directed etc.ģ. ZTE are accelerating it's global commercial deployment of the best Full-Screen UDC Smartphones, the Axon 30 and Axon 40 Ultra. So far I can tell you this is what I know and have done so far.ġ.To ensure it is not the Pc & that it is virus free, I have completely formatted (high level format not just a standard format) then reloaded with a fresh instal of win 7 + all updates.Ģ. ZTE is the global leader in telecommunications with a vision towards tommorow and is committed to creating tommorows technology, today. I am a computer/electronics tech with almost 20 years experience so I am not a noob but this is about o aggravate me. this has steadily gotten worse over time I can no longer transfer pics and files due to this I have tried everything I know please help. #Joinme zte drivers#Then, three devices will appear in the device manager.I think that it is probably 301Z in the middle, but find the one with double click → details → the end of the hardware ID is MI01.General → Update Driver → Search for driver software by referring to the computer → on the computer to select from a list of page → Show all devices → Disk → C: Program Files ( ) ZTE Join Me Drivers → CTZTE3GPhoneDiagInterface → Press 'Yes' then 'OK' or 'OK' depending on whether there is no compatibility.I have a ZTE Majesty phone model z796c android v 4.1.2 my problem is most ofthe time but not every time I connect this phone with any Usb cable (the phone will charge) but it shows the message "Usb device not recognized" when connected to my win 7 pc. JoinMe is a free PC suite designed for ZTE Phones that enables you to manage contacts, applications, messages, call log and media content. #Joinme zte how to#How to connect 301z to your PC by manuallyEven if you connect the 301Z to the PC with USB, it is inconvenient that you can not see the contents of the SD card because there is no driver.Download and install PC Connection Suite - of The driver will be expanded to C: Program Files ZTE Join Me DriversConnect 301Z to the PC. Please i Need ZTE 301Z Driver Anybody will be Appropriated TnxUser NameRemember Me?Password. Welcome to the GSM-Forum forums.You are currently viewing our boards as a guest which gives you limited access to view most discussions and access our other features.Only registered members may post questions, contact other members or search our database of over 8 million posts.Registration is fast, simple and absolutely free so please -!If you have any problems with the registration process or your account login, please contact. from January 2007 to February 2007 last updated posted 2007-Feb-2, 7:27 am AEST posted 2007-Feb-2, 7. |
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